in vitro culture method  (MetaCell Inc)

Journal: American journal of advanced drug delivery

Article Title: Artificial T Cell Mimetics to Combat Melanoma Tumor Growth

doi:

Figure Lengend Snippet: In vitro validation of hPDMP5.5. (a) Tumor sphere formation in A375, human melanoma cell line treated with PDMPs and controls IgG1 MPs and anti-hPD-1 at 50 μg/mL respectively (b) Relative number of tumor spheres. N=3 samples per condition were used for this experiment

Article Snippet: Melanoma Cell Lines and In vitro Culture Methods Human melanoma A375 and mouse B16-F10 melanoma cell lines were obtained from the American Type Culture Collection (ATCC).

Techniques: In Vitro, Biomarker Discovery

Journal: Inflammatory Bowel Diseases

Article Title: Spironolactone and colitis: Increased mortality in rodents and in humans

doi: 10.1002/ibd.21929

Figure Lengend Snippet: (A) Spironolactone inhibits TGFβ induction of αSMA protein expression in colonic myofibroblasts. A representative Western blot of αSMA expression in protein extracts from CCD-18co colonic myofibroblasts stimulated for 24 h with TGFβ is shown. Increasing amounts of spironolactone (SPIR) from 10 μM to 100 μM reduce αSMA expression to levels comparable to unstimulated cells (no Tx). GAPDH expression serves as the loading control for the amount of protein. (B) Treatment of TGFβ stimulated CCD-18co cells with spironolactone (SPIR) represses expression of Acta2, Col1a1, and Ctgf. A metabolite of spironolactone, canrenone (CAN) partially represses pro-fibrotic gene expression. (C) The role of the RAAS pathway in fibrosis and the relationship of pathway inhibitors. (D) Inhibitors of the RAAS pathway aliskiren (ALK), enalaprilat (ENT), and losartan (LOR) partially repress TGFβ induction of fibrotic genes with partial repression of Acta2 expression but have minimal effect on Col1a1 expression (E). Results are from 9 independent experiments. Asterisks denote statistically significant comparisons between untreated control cells (no Tx) and the treatment groups. Brackets denote comparisons between TGFβ treated and other treatment groups. * P <0.05, *** P <0.001

Article Snippet: In vitro myofibroblast culture methods Early passage (3 to 12) colonic human fibroblast CCD-18Co cells (CRL-1459 from ATCC) were cultured in alpha-MEM (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum and sub-cultured weekly.

Techniques: Expressing, Western Blot, Control, Gene Expression